Who takes bio here can panic wif me?
Zomg i was always not good at bio cos too much to memorise.
panic!
ExamWorld has zero Bio... how how how!!!
I take Bio too. I haven't studied Bio since my midyears, don't worry haha.
don't need panic 1 o.o
I shall panic with you.
Let's panic.. at the disco.
all who not using bio as R5 pls do mankind a favour and fail your bio to lower the bell curve thanks.
I share with everyone my best study tip
on the day of the exam, don't do any questions. Just read your TYS (must have worked solutions as well) enough.
Originally posted by eagle:I share with everyone my best study tip
on the day of the exam, don't do any questions. Just read your TYS (must have worked solutions as well) enough.
serious bo
Originally posted by davidche:serious bo
but die die must have worked solutions
sounds risky, but I think it is the best way for just before exams...
of course, today is your do papers day.... Tmr bio paper then do this method
Originally posted by eagle:I share with everyone my best study tip
on the day of the exam, don't do any questions. Just read your TYS (must have worked solutions as well) enough.
bio is new syllabus right? since eagle himself advised so, i think i will spend 5 bucks to buy worked solutions book and read for half a day.
Originally posted by eagle:but die die must have worked solutions
sounds risky, but I think it is the best way for just before exams...
of course, today is your do papers day.... Tmr bio paper then do this method
need to sleep first. evening!
Originally posted by davidche:bio is new syllabus right? since eagle himself advised so, i think i will spend 5 bucks to buy worked solutions book and read for half a day.
Worked solutions book got so cheap? :( I buy physics 1001 MCQ question with explanation already cost me $15. T.T
Originally posted by Occam's Razor:don't need panic 1 o.o
need panic twice.. LAME!~
Originally posted by iamapebble:Worked solutions book got so cheap? :( I buy physics 1001 MCQ question with explanation already cost me $15. T.T
dyna tys just the worked answers. At least they have for chem and math. Not sure bout bio XD
Originally posted by davidche:bio is new syllabus right? since eagle himself advised so, i think i will spend 5 bucks to buy worked solutions book and read for half a day.
new syllabus a not I dunno
I only know I did this method during my 1999 O levels :D
But my TYS was bought long ago, not the day before exams -.-"
Originally posted by iamapebble:Worked solutions book got so cheap? :( I buy physics 1001 MCQ question with explanation already cost me $15. T.T
physics MCQ not yet right? Chiong TYS MCQs.
I think about 4 questions will come out repeat.
for chem cos my teacher dam On, he gave us 300 mcqs the next day after chem Os cos he says mcq is impt.
Oh great, I'm dead.
I've yet to examine in detail how a fermenter works. Nor am I well-versed in the ecological chapters.
Plus, I'm still unsure of how bacteria is used as 'vectors'...
what is a fermenter? yo new syllabus?
Yes, and you're old syllabus?
i am new syllabus how come i dont rmb fermenter? what chapter?
Chapter on using bacteria to mass produce insulin.
woah you have the same syllabus but you don't know what each other is talking bout.. die already lah.. LOLs.. i never never ever pass my bio :(
Originally posted by Vendettus:Oh great, I'm dead.
I've yet to examine in detail how a fermenter works. Nor am I well-versed in the ecological chapters.
Plus, I'm still unsure of how bacteria is used as 'vectors'...
Production of insulin by bacteria using genetic engineering
• Isolation of the gene of interest
• The gene that produces insulin is identified on the human DNA.
• Using restriction enzymes, this gene is ‘cut’ from the DNA
• A fragment of the DNA with sticky ends is produced. This fragment includes the insulin gene.
• Isolation of plasmid DNA from bacterial cell
• The plasmid DNA is isolated from the bacterial cell.
• Then, using the same restriction enzyme, the plasmid DNA is cut to produce a linear strand of plasmid DNA with sticky ends.
• Insertion of the human DNA into the plasmid DNA
• The cut human DNA fragment with the gene of interest (from [1]) is mixed with the cut plasmid DNA (from [2]).
• Using enzymes to join the sticky ends, the human DNA is inserted into the plasmid DNA. ie. A recombinant DNA is produced.
• Introduce recombinant DNA back into the bacterial cell
• The bacterium is treated so that the plasmid can enter the bacterial cell.
• Thus the plasmid acts as a vector to carry the human gene into the bacterium.
• Once this is successful, the bacterium will multiply and all the bacteria produced will have the human insulin gene in them.
• Each bacterial cell will start to make insulin molecule.